Scientist, RNA-Protein Biochemistry

Location: Cambridge, Massachusetts, United States
Salary: US$45 - US$50 per hour + competitive
Sectors: R&D
Job Type: Contract
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Scientist, RNA-Protein Biochemistry - Cambridge, MA

Shift Details: Monday, Tuesday off, Wednesday half day, Thurs & Friday 12 hour shifts. Sat & Sunday half days

1 year contract

Job Description:

A well-known Pharma company is looking for a motivated individual to join their Small Molecular Physical Biochemistry group. This group functions within the company's larger Biotherapeutic and Medicinal Sciences department. The key responsibilities of this role include: expressing and purifying proteins, protein-protein and protein-RNA complexes from E.coli, insect and/or mammalian cells. The ideal candidates will also have the ability to carry out construct design, cloning, execute small (1mg) and large-scale (20mg) purifications independently. This is an amazing opportunity to work alongside like-minded individuals, in a fast faced environment, while innovatively solving unmet medical needs in the neuroscience space. Candidates who have the ability to use innovative approaches to solve problems will be highly valued.

Key Qualifications/Responsibilities:

  • Working knowledge of construct design and basic molecular biology (PCR & cloning)
  • Execute expression and purification of proteins from E. coli, insect or mammalian hosts.
  • Prior biochemistry experience in protein-protein, protein-DNA or protein-RNA complexes and their interactions will be a valuable asset.
  • Protein purification experience using Akta Express, Avant or equivalent protein purification instruments and possess experience establishing purification workflow in a RNAse free environment.
  • Independently follow established protocols for multi-step purifications and exhibit independence in protein purification. The candidate should also possess the scientific understanding behind the protocols and be able to modify them when the need arises.
  • The candidate should have proven experience in carrying out simple biophysical or biochemical experiments to evaluate protein-nucleic acid interactions.
  • Ability to trouble shoot and solve scientific and technical problems with minimal supervision.
  • Ability to document all aspects of experiments performed.

Expected Education/Experience:

  • Masters (and 3-4 years of relevant experience in an industrial or academic lab) or Ph.D degrees in Biophysics, Biochemistry or related discipline are required.
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